The PacBio chemistries¶
PacBio sequencing generate output formats that are different depending on the chemistry used in the sequencing. The change in the chemistries are because these undergo improvements to produce better results, however this also have produced a data incompatibility when processing or comparisons are made between different chemistries.
These are some of the chemistries used by PacBio and they are related to the versions of the kits used to process the DNA.
Chemistry table¶
BindingKit |
SequencingKit |
Chemistry |
System |
|---|---|---|---|
100-356-300 |
100-356-200 |
P6-C4 |
RS |
100-356-300 |
100-356-200 |
P6-C4 |
RS |
100-356-300 |
100-612-400 |
P6-C4 |
RS |
100-356-300 |
100-612-400 |
P6-C4 |
RS |
100-372-700 |
100-356-200 |
P6-C4 |
RS |
100-372-700 |
100-356-200 |
P6-C4 |
RS |
100-372-700 |
100-612-400 |
P6-C4 |
RS |
100-372-700 |
100-612-400 |
P6-C4 |
RS |
100-619-300 |
100-620-000 |
S/P1-C1/beta |
RS? |
100-619-300 |
100-620-000 |
S/P1-C1/beta |
RS? |
100-619-300 |
100-867-300 |
S/P1-C1.1 |
RS? |
100-619-300 |
100-867-300 |
S/P1-C1.1 |
RS? |
100-619-300 |
100-867-300 |
S/P1-C1.1 |
RS? |
100-619-300 |
100-902-100 |
S/P1-C1.2 |
RS? |
100-619-300 |
100-902-100 |
S/P1-C1.2 |
RS? |
100-619-300 |
100-902-100 |
S/P1-C1.2 |
RS? |
100-619-300 |
100-902-100 |
S/P1-C1.2 |
RS? |
100-619-300 |
100-902-100 |
S/P1-C1.2 |
RS? |
100-619-300 |
100-972-200 |
S/P1-C1.3 |
RS? |
100-619-300 |
100-972-200 |
S/P1-C1.3 |
RS? |
100-619-300 |
100-972-200 |
S/P1-C1.3 |
RS? |
100-619-300 |
100-972-200 |
S/P1-C1.3 |
RS? |
100-862-200 |
101-093-700 |
S/P2-C2/5.0 |
Sequel |
100-862-200 |
100-861-800 |
S/P2-C2/5.0 |
Sequel |
100-862-200 |
101-309-400 |
S/P2-C2/5.0 |
Sequel |
100-862-200 |
101-309-500 |
S/P2-C2/5.0 |
Sequel |
101-365-900 |
100-861-800 |
S/P2-C2/5.0 |
Sequel |
101-365-900 |
101-093-700 |
S/P2-C2/5.0 |
Sequel |
101-365-900 |
101-309-400 |
S/P2-C2/5.0 |
Sequel |
101-365-900 |
101-309-500 |
S/P2-C2/5.0 |
Sequel |
101-500-400 |
101-427-500 |
S/P3-C3/5.0 |
Sequel |
101-500-400 |
101-427-800 |
S/P3-C3/5.0 |
Sequel |
101-500-400 |
101-646-800 |
S/P3-C3/5.0 |
Sequel |
101-490-800 |
101-490-900 |
S/P3-C1/5.0-8M |
Sequel II |
101-490-800 |
101-491-000 |
S/P3-C1/5.0-8M |
Sequel II |
101-490-800 |
101-644-500 |
S/P3-C1/5.0-8M |
Sequel II |
101-490-800 |
101-717-100 |
S/P3-C1/5.0-8M |
Sequel II |
101-717-300 |
101-644-500 |
S/P3-C1/5.0-8M |
Sequel II |
101-717-300 |
101-717-100 |
S/P3-C1/5.0-8M |
Sequel II |
101-717-400 |
101-644-500 |
S/P3-C1/5.0-8M |
Sequel II |
101-717-400 |
101-717-100 |
S/P3-C1/5.0-8M |
Sequel II |
101-789-500 |
101-789-300 |
S/P4-C2/5.0-8M |
Sequel II |
101-820-500 |
101-789-300 |
S/P4.1-C2/5.0-8M |
Sequel II |
101-789-500 |
101-826-100 |
S/P4-C2/5.0-8M |
Sequel II |
101-789-500 |
101-820-300 |
S/P4-C2/5.0-8M |
Sequel II |
101-820-500 |
101-826-100 |
S/P4.1-C2/5.0-8M |
Sequel II |
101-820-500 |
101-820-300 |
S/P4.1-C2/5.0-8M |
Sequel II |
101-894-200 |
101-826-100 |
S/P5-C2/5.0-8M |
Sequel II |
101-894-200 |
101-789-300 |
S/P5-C2/5.0-8M |
Sequel II |
101-894-200 |
101-820-300 |
S/P5-C2/5.0-8M |
Sequel II |
Adapted from on: * Reference 1 * Reference 2 * Reference 3
Chemistries in our data¶
Name |
BindingKit |
SequencingKit |
Chemistry |
Platform |
|---|---|---|---|---|
pMA683 |
100356300 |
100612400 |
P6-C4 |
RS |
pMA684 |
100356300 |
100612400 |
P6-C4 |
RS |
pMA685 |
100356300 |
100612400 |
P6-C4 |
RS |
OriC_enrichment |
100-862-200 |
100-861-800 |
P2-C2 |
SEQUEL |
1_A07-50pM |
101-500-400 |
101-427-800 |
P3-C3 |
SEQUEL |
4_D05-10pM |
101-500-400 |
101-427-800 |
P3-C3 |
SEQUEL |
2_B07-20pM |
101-500-400 |
101-427-800 |
P3-C3 |
SEQUEL |
1_A09->100pM |
101-500-400 |
101-427-800 |
P3-C3 |
SEQUEL |