Overview

This project is about detecting modifications of DNA in PacBio sequencing data stored in the BAM format. The PacBio sequencing data is processed at molecule level: each molecule/ZMW/hole number is analyzed individually.

To learn how to start using this software, have a look at the quickstart section.

Or check the usage section, for more details.

• Free software: GNU General Public License v3

• Documentation: https://pacbio-data-processing.readthedocs.io

• Gitlab repository: https://gitlab.com/dvelazquez/pacbio-data-processing

Features

• Detection of individual m6A modifications in each molecule.

• The single molecule analysis of a BAM file can be performed with a command line interface (CLI) program sm-analysis or using a simple graphical user interface (GUI) program, sm-analysis-gui.

• The single molecule analysis can be parallelized to speed up the processing of large BAM files.

• An auxiliary executable, bam-filter, is also provided.

Credits

This package was created with Cookiecutter and the palao/cookiecutter-genrepo-pypackage project template.