The PacBio chemistries ---------------------- PacBio sequencing generate output formats that are different depending on the chemistry used in the sequencing. The change in the chemistries are because these undergo improvements to produce better results, however this also have produced a data incompatibility when processing or comparisons are made between different chemistries. These are some of the chemistries used by PacBio and they are related to the versions of the kits used to process the DNA. Chemistry table ~~~~~~~~~~~~~~~ =========== ============= ================ ========= BindingKit SequencingKit Chemistry System =========== ============= ================ ========= 100-356-300 100-356-200 P6-C4 RS 100-356-300 100-356-200 P6-C4 RS 100-356-300 100-612-400 P6-C4 RS 100-356-300 100-612-400 P6-C4 RS 100-372-700 100-356-200 P6-C4 RS 100-372-700 100-356-200 P6-C4 RS 100-372-700 100-612-400 P6-C4 RS 100-372-700 100-612-400 P6-C4 RS 100-619-300 100-620-000 S/P1-C1/beta RS? 100-619-300 100-620-000 S/P1-C1/beta RS? 100-619-300 100-867-300 S/P1-C1.1 RS? 100-619-300 100-867-300 S/P1-C1.1 RS? 100-619-300 100-867-300 S/P1-C1.1 RS? 100-619-300 100-902-100 S/P1-C1.2 RS? 100-619-300 100-902-100 S/P1-C1.2 RS? 100-619-300 100-902-100 S/P1-C1.2 RS? 100-619-300 100-902-100 S/P1-C1.2 RS? 100-619-300 100-902-100 S/P1-C1.2 RS? 100-619-300 100-972-200 S/P1-C1.3 RS? 100-619-300 100-972-200 S/P1-C1.3 RS? 100-619-300 100-972-200 S/P1-C1.3 RS? 100-619-300 100-972-200 S/P1-C1.3 RS? 100-862-200 101-093-700 S/P2-C2/5.0 Sequel 100-862-200 100-861-800 S/P2-C2/5.0 Sequel 100-862-200 101-309-400 S/P2-C2/5.0 Sequel 100-862-200 101-309-500 S/P2-C2/5.0 Sequel 101-365-900 100-861-800 S/P2-C2/5.0 Sequel 101-365-900 101-093-700 S/P2-C2/5.0 Sequel 101-365-900 101-309-400 S/P2-C2/5.0 Sequel 101-365-900 101-309-500 S/P2-C2/5.0 Sequel 101-500-400 101-427-500 S/P3-C3/5.0 Sequel 101-500-400 101-427-800 S/P3-C3/5.0 Sequel 101-500-400 101-646-800 S/P3-C3/5.0 Sequel 101-490-800 101-490-900 S/P3-C1/5.0-8M Sequel II 101-490-800 101-491-000 S/P3-C1/5.0-8M Sequel II 101-490-800 101-644-500 S/P3-C1/5.0-8M Sequel II 101-490-800 101-717-100 S/P3-C1/5.0-8M Sequel II 101-717-300 101-644-500 S/P3-C1/5.0-8M Sequel II 101-717-300 101-717-100 S/P3-C1/5.0-8M Sequel II 101-717-400 101-644-500 S/P3-C1/5.0-8M Sequel II 101-717-400 101-717-100 S/P3-C1/5.0-8M Sequel II 101-789-500 101-789-300 S/P4-C2/5.0-8M Sequel II 101-820-500 101-789-300 S/P4.1-C2/5.0-8M Sequel II 101-789-500 101-826-100 S/P4-C2/5.0-8M Sequel II 101-789-500 101-820-300 S/P4-C2/5.0-8M Sequel II 101-820-500 101-826-100 S/P4.1-C2/5.0-8M Sequel II 101-820-500 101-820-300 S/P4.1-C2/5.0-8M Sequel II 101-894-200 101-826-100 S/P5-C2/5.0-8M Sequel II 101-894-200 101-789-300 S/P5-C2/5.0-8M Sequel II 101-894-200 101-820-300 S/P5-C2/5.0-8M Sequel II =========== ============= ================ ========= Adapted from on: \* `Reference 1 `__ \* `Reference 2 `__ \* `Reference 3 `__ Chemistries in our data ~~~~~~~~~~~~~~~~~~~~~~~ =============== =========== ============= ========= ======== Name BindingKit SequencingKit Chemistry Platform =============== =========== ============= ========= ======== pMA683 100356300 100612400 P6-C4 RS pMA684 100356300 100612400 P6-C4 RS pMA685 100356300 100612400 P6-C4 RS OriC_enrichment 100-862-200 100-861-800 P2-C2 SEQUEL 1_A07-50pM 101-500-400 101-427-800 P3-C3 SEQUEL 4_D05-10pM 101-500-400 101-427-800 P3-C3 SEQUEL 2_B07-20pM 101-500-400 101-427-800 P3-C3 SEQUEL 1_A09->100pM 101-500-400 101-427-800 P3-C3 SEQUEL =============== =========== ============= ========= ========